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KMID : 0880220110490050753
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Journal of Microbiology
2011 Volume.49 No. 5 p.753 ~ p.758
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Production of cephalosporin C using crude glycerol in fed-batch culture of Acremonium chrysogenum M35
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Shin Hyun-Yong
Lee Jin-Young
Choi Han-Suk
Lee Ja-Hyun
Kim Seung-Wook
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Abstract
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In this study, cephalosporin C production by Acremonium chrysogenum M35 cultured with crude glycerol instead of rice oil and methionine was investigated. The addition of crude glycerol increased cephalosporin C production by 6-fold in shake-flask culture, and also the amount of cysteine. In fed-batch culture without methionine, crude glycerol resulted only in overall improvement in cephalosporin C production (about 700%). In addition, A. chrysogenum M35 became highly differentiated in fed-batch culture with crude glycerol, compared with the differentiation in batch culture. The results presented here suggest that crude glycerol can replace methionine and plant oil as cysteine and carbon sources during cephalosporin C production by A. chrysogenum M35.
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KEYWORD
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cysteine, cystathionine-γ-lyase activity, Acremonium chrysogenum M35, methionine, cephalosporin C, crude glycerol
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